Sterilization with ETO is a low-temperature process (typically between 30 and 70°C) that uses Ethylene Oxide gas or ethylene chlorohydrin to reduce the level of infectious agents. ETO is used in gas form and is usually mixed with other substances, such as CO2 or steam. It is mainly used for products that cannot withstand the heat of typical autoclave sterilization such as plastic. The main physical parameters to be monitored are temperature and relative humidity.
The total length of the process can vary depending on the goods that are being sterilized, but typical cycles are between 36 and 48 hours. There are two main ways to divide the cycle; a three phase cycle and a five phase cycle. The difference being that a three phase cycle does factor in the pre and post exposure phases.
The final process is ETO gas is removed using changes of air in the sterilization chamber removing any ETO particles.
Steam sterilization is an perfect method as it is effective and natural. The process has become very common treatment of herbs, spices.
Specific temperatures must be obtained to ensure the microbicidal activity. The two common steam-sterilizing temperatures are 121°C (250°F) and 132°C (270°F). These temperatures (and other high temperatures)830 must be maintained for a minimal time to kill microorganisms.
Steam sterilization is a technological process involving the destruction of microorganisms with the concurrent preservation of organoleptic properties of raw materials.
The sterilizing factor is saturated steam in overpressure, with a temperature from 121 to 390˚C. Steam sterilization achieves a full effect if steam affects the commodity undergoing sterilization over a strictly set time, from 15 to 60 seconds.
After the sterilization process product color change